3FM7
Quaternary Structure of Drosophila melanogaster IC/Tctex-1/LC8; Allosteric Interactions of Dynein Light Chains with Dynein Intermediate Chain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .98 |
| Spacegroup name | P 62 |
| Unit cell lengths | 115.790, 115.790, 90.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 3.500 |
| R-factor | 0.18001 |
| Rwork | 0.170 |
| R-free | 0.27470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | chains A and F in pdb structure 2PG1 |
| RMSD bond length | 0.000 |
| RMSD bond angle | 0.047 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.640 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.088 | 0.358 |
| Number of reflections | 8745 | |
| <I/σ(I)> | 15.9 | 6.2 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 7.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Hanging Drop | 6.5 | 277.15 | 16% PEG 8K, 100 mM sodium cacodylate, 200 mM calcium acetate, pH 6.5, Hanging Drop, temperature 277.15K |
