3FM5
X-ray crystal structure of transcriptional regulator (MarR family) from Rhodococcus sp. RHA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 75.114, 75.114, 213.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.136 - 2.000 |
R-factor | 0.1964 |
Rwork | 0.194 |
R-free | 0.23980 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.706 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.085 | 0.603 |
Number of reflections | 44238 | |
<I/σ(I)> | 23.7 | 3 |
Completeness [%] | 97.7 | 91.9 |
Redundancy | 12.2 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M Mg(OAC), 20% PEG 3350, 4% Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |