3FLT
Crystal structure of PE-bound octameric SAP-like pentraxin from Limulus polyphemus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 172.390, 172.390, 98.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.680 - 2.700 |
| R-factor | 0.243 |
| Rwork | 0.210 |
| R-free | 0.24300 |
| Starting model (for MR) | Crystals were isomorphous to octameric native. Native structure pdb code 3FLR was used in rigid body refinement. |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.1.4) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.681 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.084 | 0.252 |
| Total number of observations | 11379 | |
| Number of reflections | 20530 | |
| <I/σ(I)> | 4.975 | 2.6 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG 6000, CaCl2, MES, PE, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
