3FLR
Crystal structure of native octameric SAP-like pentraxin from Limulus polyphemus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 298 |
| Detector technology | FILM |
| Collection date | 1989-01-01 |
| Detector | CEA |
| Wavelength(s) | 1.488 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 173.330, 173.330, 98.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.900 - 3.000 |
| R-factor | 0.214 |
| Rwork | 0.184 |
| R-free | 0.23200 |
| Starting model (for MR) | Rigid body refinement of polyalanine model of native octameric Limulus SAP PDB code 1qtj |
| Data reduction software | MOSFLM |
| Data scaling software | ROTAVATA/AGROVATA |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.900 | 3.130 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.117 | 0.284 |
| Number of reflections | 13666 | |
| Completeness [%] | 89.0 | 55.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 298 | Tris, NaCl, pH 7.4, sitting drop, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
