3FLK
Crystal Structure of Tartrate Dehydrogenase from Pseudomonas putida in complex with NADH, oxalate and metal ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-07-24 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 117.278, 117.278, 291.324 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.20862 |
Rwork | 0.208 |
R-free | 0.22692 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.025 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.110 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 126431 | |
<I/σ(I)> | 21.4 | 4.97 |
Completeness [%] | 99.0 | 90 |
Redundancy | 12.2 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.75 M ammonium sulfate, 0.2 M potassium acetate, 0.02 M DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |