3FLH

Crystal structure of lp_1913 protein from Lactobacillus plantarum,Northeast Structural Genomics Consortium Target LpR140B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-02
Detector	MAR CCD 165 mm
Wavelength(s)	0.97892
Spacegroup name	P 1 21 1
Unit cell lengths	57.892, 47.429, 62.769
Unit cell angles	90.00, 113.55, 90.00

Refinement procedure

Resolution	19.880 - 2.000
R-factor	0.195
Rwork	0.193
R-free	0.24800
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELX (then SOLVE/RESOLVE)
Refinement software	CNS (1.2 & XtalView)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.103	0.371
Number of reflections	41366
<I/σ(I)>	16.52	4.5
Completeness [%]	99.7	100
Redundancy	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	293	Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, and 5 mM DTT. Reservoir solution: 100 mM sodium acetate (pH 5.0), 40% PEG 4k, and 100 mM sodium bromide. Microbatch under oil, temperature 293K