3FLD
Crystal structure of the trai c-terminal domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2006-12-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97835, 0.97873, 0.97126 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 40.800, 139.751, 126.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.26300 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.270 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 24509 | |
<I/σ(I)> | 5.4 | |
Completeness [%] | 89.4 | 89.4 |
Redundancy | 6.1 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 1 M ammonium sulfate, 0.1 M MES, pH 6.0, cryoprotected in 1.8 M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |