3FKS
Yeast F1 ATPase in the absence of bound nucleotides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 111.934, 290.487, 188.700 |
| Unit cell angles | 90.00, 101.75, 90.00 |
Refinement procedure
| Resolution | 33.203 - 3.587 |
| R-factor | 0.2435 |
| Rwork | 0.242 |
| R-free | 0.30620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w0j |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.800 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.690 |
| High resolution limit [Å] | 3.587 | 3.587 |
| Rmerge | 0.094 | 0.423 |
| Number of reflections | 134821 | |
| <I/σ(I)> | 9 | 1.6 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | 6.25% PEG 6000, 10% Glycerol, 4% Methanol, 0.05M Sodium acetate, 0.5mM Nickel sulfate, 2 mM Sodium pyrophosphate, 2mM Magnesium chloride, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
