3FKH

Crystal structure of Putative pyridoxamine 5'-phosphate oxidase (NP_601736.1) from CORYNEBACTERIUM GLUTAMICUM ATCC 13032 KITASATO at 2.51 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.94645,0.97962
Spacegroup name	P 21 21 21
Unit cell lengths	74.630, 88.310, 138.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.540 - 2.510
R-factor	0.245
Rwork	0.243
R-free	0.28700
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.647
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.540	29.540	2.590
High resolution limit [Å]	2.510	5.350	2.500
Rmerge	0.062	0.041	0.296
Number of reflections	31540	6155	4321
<I/σ(I)>	15.98	33.2	2
Completeness [%]	96.0	97.8	69.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.2000M Ca(OAc)2, 20.0000% PEG-8000, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K