3FKC
Crystal Structure of Human Zinc finger and BTB domain containing 33
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 118.358, 118.358, 38.981 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.980 - 1.700 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2VKP 2if5 2nn2 2ihc 1buo 1cs3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.450 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.2) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.984 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.057 | 0.664 |
| Number of reflections | 18142 | |
| <I/σ(I)> | 27.1 | 3 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 10.2 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 277 | 0.8M (NH4)2SO4 0.1M citrate pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
