3FK3

Structure of the Yeats Domain, Yaf9

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	298
Detector technology	CCD
Collection date	2004-06-15
Detector	ADSC QUANTUM 210
Wavelength(s)	1.11587, 0.9795, 1.0199
Spacegroup name	P 65 2 2
Unit cell lengths	84.725, 84.725, 288.212
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.22394
Rwork	0.222
R-free	0.25746
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.989
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.420
High resolution limit [Å]	2.300	2.300
Number of reflections	24936
Completeness [%]	95.8	92.8
Redundancy	6.3	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	291	10% PEG 3350, 100 mM Na, Tartrate, 20% glycerol, pH 7.5, microbatch, temperature 291K