3FJ5
Crystal structure of the c-src-SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-03-03 |
| Detector | Mar CCD-165 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 65 |
| Unit cell lengths | 46.596, 46.596, 128.064 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hda |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.029 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.357 | 40.360 | 1.690 |
| High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
| Rmerge | 0.078 | 0.064 | 0.543 |
| Total number of observations | 3858 | 20221 | |
| Number of reflections | 19644 | ||
| <I/σ(I)> | 4.701 | 8.8 | 1.4 |
| Completeness [%] | 95.6 | 90.3 | 95 |
| Redundancy | 7.1 | 6.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 288 | 1.7M Ammonium sulphate, 5% PEG300, 10% glycerol, 0.1M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
