3FIW
Structure of SCO0253, a Tetr-family transcriptional regulator from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.487, 114.352, 67.446 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 42.800 - 2.200 |
| R-factor | 0.19243 |
| Rwork | 0.189 |
| R-free | 0.25550 |
| Structure solution method | SAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.703 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 (ShelxC) |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 2.220 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.114 | |
| Number of reflections | 48607 | |
| <I/σ(I)> | 26.18 | 4 |
| Completeness [%] | 97.4 | 83.8 |
| Redundancy | 7.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M Sodium Cacodylate ph 6.5, 0.2 M Ammonium Sulfate, 30% PEG 8K, cryoprotected with N-paratone oil, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
