3FIP
Crystal structure of Usher PapC translocation pore
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2008-04-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97931 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 120.036, 120.036, 354.151 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.679 - 3.154 |
R-factor | 0.289 |
Rwork | 0.285 |
R-free | 0.35900 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.370 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 41032 | |
<I/σ(I)> | 34.8 | 2.4 |
Completeness [%] | 96.3 | |
Redundancy | 6.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.9 | 293 | 3.3M NaCl, 0.06%LDAO, 4% 2,5 hexanidiol, 20mM Tris, pH 4.9, EVAPORATION, temperature 293K |