3FIJ
Crystal structure of a uncharacterized protein lin1909
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.056, 131.205, 93.290 |
Unit cell angles | 90.00, 108.79, 90.00 |
Refinement procedure
Resolution | 43.100 - 2.300 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.26900 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.130 | 0.656 |
Number of reflections | 91961 | |
<I/σ(I)> | 10.8 | 2 |
Completeness [%] | 99.7 | 98.5 |
Redundancy | 7 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M MES 6.5, 12% PEG 20,000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |