3FIB
RECOMBINANT HUMAN GAMMA-FIBRINOGEN CARBOXYL TERMINAL FRAGMENT (RESIDUES 143-411) BOUND TO CALCIUM AT PH 6.0: A FURTHER REFINEMENT OF PDB ENTRY 1FIB, AND DIFFERS FROM 1FIB BY THE MODELLING OF A CIS PEPTIDE BOND BETWEEN RESIDUES K338 AND C339
Experimental procedure
| Temperature [K] | 300 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-08 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.530, 68.420, 47.670 |
| Unit cell angles | 90.00, 105.36, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.154 |
| Rwork | 0.154 |
| R-free | 0.20600 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.770 |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.000 |
| High resolution limit [Å] | 2.030 |
| Rmerge | 0.033 |
| Number of reflections | 12334 |
| Completeness [%] | 82.7 |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 | 18% PEG 8000, 0.1 M MES PH 6.0, 70MM CACL2, SITTING DROP VAPOR DIFFUSION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | (%) | |
| 2 | 1 | reservoir | (mM) | ||
| 3 | 1 | reservoir | MES | (M) | |
| 4 | 1 | reservoir | (%) | ||
| 5 | 1 | drop | protein | (mg/ml) | |
| 6 | 1 | drop | reservoir solution |
