3FI9
Crystal structure of malate dehydrogenase from Porphyromonas gingivalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97958 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.385, 74.133, 135.296 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.254 |
Rwork | 0.252 |
R-free | 0.29500 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.467 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.937 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.112 | 0.378 |
Total number of observations | 55200 | |
Number of reflections | 56890 | |
<I/σ(I)> | 9 | 3.2 |
Completeness [%] | 99.3 | 98 |
Redundancy | 7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 294 | 20% PEG 3350, 200mM ammonium di-tartrate, pH 7.0, vapor diffusion, temperature 294K |