3FI7
Crystal Structure of the autolysin Auto (Lmo1076) from Listeria monocytogenes, catalytic domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.934 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 133.500, 133.500, 88.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.480 - 2.350 |
R-factor | 0.181 |
Rwork | 0.180 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.883 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 19770 | |
<I/σ(I)> | 37.71 | 6.49 |
Completeness [%] | 99.2 | 100 |
Redundancy | 15.9 | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1M ammonium sulfate, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |