3FI0
Crystal Structure Analysis of B. stearothermophilus Tryptophanyl-tRNA Synthetase Complexed with Tryptophan, AMP, and Inorganic Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-15 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97934,0.97945,0.97117 |
| Spacegroup name | P 1 |
| Unit cell lengths | 122.032, 122.396, 122.341 |
| Unit cell angles | 79.90, 80.52, 79.81 |
Refinement procedure
| Resolution | 25.000 - 2.700 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22000 |
| RMSD bond length | 0.044 |
| RMSD bond angle | 3.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.069 |
| Number of reflections | 203226 |
| Completeness [%] | 97.7 |
| Redundancy | 3.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.58 | 315 | 2.18 M K2HPO4, 0.6% (v/v) PEG 400, pH 6.58, dialysis, temperature 315K |
