3FH4

Crystal Structure of Recombinant Vibrio proteolyticus aminopeptidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	2008-08-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	108.397, 108.397, 93.529
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.030 - 1.950
R-factor	0.19294
Rwork	0.191
R-free	0.22776
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2iq6
RMSD bond length	0.009
RMSD bond angle	1.083
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.890
High resolution limit [Å]	1.950	1.950
Number of reflections	23158
<I/σ(I)>		31.5
Completeness [%]	100.0	100
Redundancy	21	21

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	VpAP (0.5 mM) in 10 mM Tris buffer, pH 8.0, containing 10 mM KSCN and 400 mM NaCl was equilibrated with 100 mM Tris buffer, pH 8.0, containing 100 mM KSCN and 4.5 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K