3FH2
The crystal structure of the PROBABLE ATP-DEPENDENT PROTEASE (HEAT SHOCK PROTEIN) from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.231, 60.830, 68.767 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.380 - 1.600 |
| R-factor | 0.19109 |
| Rwork | 0.189 |
| R-free | 0.23105 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.792 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.380 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.317 |
| Number of reflections | 17893 | |
| <I/σ(I)> | 30.1 | 2.69 |
| Completeness [%] | 99.2 | 91.63 |
| Redundancy | 8.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 25.5% PEG4000, 0.085M Na Citrate, 0.17M Ammonium acetate, 15% glycerol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
