3FGG
Crystal Structure of Putative ECF-type Sigma Factor Negative Effector from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-03 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.849, 63.055, 70.374 |
| Unit cell angles | 90.00, 115.52, 90.00 |
Refinement procedure
| Resolution | 31.820 - 2.300 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.639 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.820 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.128 | 0.406 |
| Number of reflections | 24479 | |
| <I/σ(I)> | 8 | 1.9 |
| Completeness [%] | 98.1 | 87.8 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2 M Zinc acetate, 0.1 M Sodium cacodylate pH 6.5, 10 %v/v Isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
