3FG8
Crystal structure of PAS domain of RHA05790
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-27 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97926 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 149.695, 55.889, 101.856 |
Unit cell angles | 90.00, 132.61, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.17074 |
Rwork | 0.168 |
R-free | 0.22259 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.547 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.076 | 0.461 |
Number of reflections | 57336 | |
<I/σ(I)> | 30.94 | 4.27 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | 0.1M Bis-tris pH6.5, 28%PEG2KMME, VAPOR DIFFUSION, HANGING DROP, temperature 297K |