3FG2
Crystal Structure of Ferredoxin Reductase for the CYP199A2 System from Rhodopseudomonas palustris
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.538, 107.538, 69.908 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| Rwork | 0.213 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q1w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.520 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.440 |
| Number of reflections | 23724 | |
| Redundancy | 7.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.1M HEPES, 16%(w/v) PEG10000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
