3FFY
Putative tetrapyrrole (corrin/porphyrin) methyltransferase from Bacteroides fragilis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.536, 60.536, 96.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.100 - 2.000 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.758 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.709 |
| Number of reflections | 11909 | |
| <I/σ(I)> | 40.087 | 2.08 |
| Completeness [%] | 94.1 | 57.4 |
| Redundancy | 9 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | 1.26 M ammonium sulfate, 0.1 M acetate buffer, 0.2 M sodium chloride, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
