3FFR

CRYSTAL STRUCTURE OF A PHOSPHOSERINE AMINOTRANSFERASE SERC (CHU_0995) FROM CYTOPHAGA HUTCHINSONII ATCC 33406 AT 1.75 A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-12
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97870,0.97821
Spacegroup name	P 31 2 1
Unit cell lengths	79.230, 79.230, 144.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.698 - 1.750
R-factor	0.144
Rwork	0.143
R-free	0.16200
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.675
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.4.0067)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.698	26.698	1.810
High resolution limit [Å]	1.750	3.760	1.750
Rmerge	0.051	0.026	0.578
Number of reflections	53599	10275	9637
<I/σ(I)>	16.32	43.7	2.3
Completeness [%]	99.6	99.5	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	5.0000% PEG-1000, 40.0000% PEG-300, 0.1M TRIS pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K