3FFO
F17b-G lectin domain with bound GlcNAc(beta1-2)man
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.8453 |
| Spacegroup name | P 62 |
| Unit cell lengths | 87.389, 87.389, 56.809 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.920 - 2.100 |
| R-factor | 0.237 |
| Rwork | 0.233 |
| R-free | 0.27100 |
| Starting model (for MR) | 1o9v |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.970 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.065 | 0.340 |
| Number of reflections | 14544 | |
| <I/σ(I)> | 21 | 6.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.8 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 1.2M lithium sulphate, 10mM nickel chloride, 100mM TRIS, pH 8.5, VAPOR DIFFUSION, temperature 293K |
