3FFN
Crystal structure of calcium-free human gelsolin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2008-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 170.887, 170.887, 152.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.840 - 3.000 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.27100 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.397 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.430 | 3.000 |
| Rmerge | 0.083 | 0.051 | 0.338 |
| Number of reflections | 45666 | ||
| <I/σ(I)> | 27.188 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 8.4 | 8.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 297 | 15% glycerol, 100 mM Bis-Tris HCl, 1.5M ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
