3FFD
Structure of parathyroid hormone-related protein complexed to a neutralizing monoclonal antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-07-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.99988 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 72.613, 96.285, 88.544 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1IGT AND 1IGC |
RMSD bond length | 0.017 |
RMSD bond angle | 1.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.083 | 0.696 |
Number of reflections | 42012 | |
<I/σ(I)> | 27.8 | 4.1 |
Completeness [%] | 98.5 | 100 |
Redundancy | 8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 295 | PEG 400, TRIS, pH 8.70, VAPOR DIFFUSION, HANGING DROP, temperature 295K |