3FFB
Gi-alpha-1 mutant in GDP bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2006-11-06 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 4 |
Unit cell lengths | 121.761, 121.761, 68.714 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.800 - 2.570 |
Rwork | 0.181 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bof |
RMSD bond length | 0.012 |
RMSD bond angle | 1.372 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.800 | 2.700 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmerge | 0.060 | 0.275 |
Number of reflections | 16157 | |
<I/σ(I)> | 7.4 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.3 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 293 | 100 mM Sodium Acetate pH 5.9-6.3, 1.8-2.1 M Ammonium Sulphate, VAPOR DIFFUSION, temperature 293K, pH 6.0 |