3FF8

Structure of NK cell receptor KLRG1 bound to E-cadherin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-07
Detector	ADSC QUANTUM 315
Wavelength(s)	1.502
Spacegroup name	P 21 21 21
Unit cell lengths	53.776, 90.526, 107.593
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
Rwork	0.212
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3FF9 and 2O72
RMSD bond length	0.011
RMSD bond angle	1.568
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.072	0.035	0.484
Number of reflections	36191
<I/σ(I)>	46.411
Completeness [%]	100.0	99.7	100
Redundancy	14.4	13.5	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	PEG8000, pH 6.5, vapor diffusion, temperature 298K