3FF5
Crystal structure of the conserved N-terminal domain of the peroxisomal matrix-protein-import receptor, Pex14p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2008-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.8 |
Spacegroup name | I 2 3 |
Unit cell lengths | 90.570, 90.570, 90.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.18104 |
Rwork | 0.179 |
R-free | 0.21081 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.023 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.076 | 0.361 |
Number of reflections | 11491 | |
<I/σ(I)> | 39.6 | 6.3 |
Completeness [%] | 100.0 | 93.2 |
Redundancy | 17.8 | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.2M sodium citrate, 100mM HEPES(pH7.5), 10%(v/v) glycerol, 0.2%(w/v) n-decylphosphocholine, VAPOR DIFFUSION, SITTING DROP, temperature 277K |