3FEZ

Crystal structure of uncharacterized ferredoxin fold protein related to antibiotic biosynthesis monooxygenases (YP_014836.1) from LISTERIA MONOCYTOGENES 4b F2365 at 2.10 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-12
Detector	MAR MAR300
Wavelength(s)	0.94645,0.97966
Spacegroup name	P 61 2 2
Unit cell lengths	73.220, 73.220, 214.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.976 - 2.100
R-factor	0.204
Rwork	0.203
R-free	0.22700
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.289
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.976		2.170
High resolution limit [Å]	2.100	4.510	2.100
Rmerge	0.155	0.011	0.011
Number of reflections	20290	3761	3205
<I/σ(I)>		24.7	1.6
Completeness [%]	97.9	97.9	91.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277	0.2000M NH4H2PO3, 20.0000% PEG-3350, No Buffer pH 4.6, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K