3FEQ
Crystal structure of uncharacterized protein eah89906
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-02 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 |
| Unit cell lengths | 113.208, 108.048, 171.132 |
| Unit cell angles | 81.75, 80.36, 74.40 |
Refinement procedure
| Resolution | 20.000 - 2.630 |
| R-factor | 0.23902 |
| Rwork | 0.238 |
| R-free | 0.28148 |
| Structure solution method | MR |
| Starting model (for MR) | 2R8C |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.206 | 0.760 |
| Number of reflections | 227280 | |
| <I/σ(I)> | 3.2 | 1 |
| Completeness [%] | 98.0 | 97.1 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 6% PEG 8000, MES, PH 6.0, 200MM SODIU ACYTATE,10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
