3FEK
Crystal structure of the R132K:Y134F:R111L:L121D:T54V mutant of cellular retinoic acid-binding protein II at 1.51 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97850 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.601, 37.189, 57.056 |
| Unit cell angles | 73.64, 76.67, 87.70 |
Refinement procedure
| Resolution | 33.660 - 1.510 |
| R-factor | 0.17579 |
| Rwork | 0.174 |
| R-free | 0.21028 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g7b |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.425 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.500 | 1.560 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.041 | 0.232 |
| Number of reflections | 39062 | |
| <I/σ(I)> | 46.2 | 4.5 |
| Completeness [%] | 93.3 | 70.5 |
| Redundancy | 4.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Tris, 0.2M NaOAc, 24% PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
