3FEF

Crystal structure of putative glucosidase lplD from bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	81.987, 84.843, 86.051
Unit cell angles	69.80, 68.10, 64.07

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.19
Rwork	0.187
R-free	0.24300
RMSD bond length	0.010
RMSD bond angle	1.249
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.070	0.174
Number of reflections	90052
<I/σ(I)>	10.697	5.1
Completeness [%]	94.0	63.8
Redundancy	2.9	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	23% PEG 3350, 0.17M Ammonium Sulphate, 0.1M Magnesium Acetate, pH 7.0, Vapor diffusion, Sitting drop, temperature 298K