3FED
The high resolution structure of human glutamate carboxypeptidase III (GCPIII/NAALADase II) in complex with a transition state analog of Glu-Glu
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-01 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.731, 104.086, 77.634 |
Unit cell angles | 90.00, 108.19, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.290 |
R-factor | 0.13104 |
Rwork | 0.131 |
R-free | 0.14727 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2or4 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.830 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.340 |
High resolution limit [Å] | 1.290 | 1.290 |
Rmerge | 0.092 | 0.469 |
Number of reflections | 227958 | |
<I/σ(I)> | 1.9 | |
Completeness [%] | 97.8 | 79.8 |
Redundancy | 6.6 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES-Na, 10% (w/v) PEG6000, 5% (v/v) MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |