3FE5
Crystal structure of 3-hydroxyanthranilate 3,4-dioxygenase from bovine kidney
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07-27 |
| Detector | MAR scanner 180 mm plate |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.250, 42.520, 46.480 |
| Unit cell angles | 91.70, 113.30, 107.70 |
Refinement procedure
| Resolution | 35.070 - 2.510 |
| R-factor | 0.16689 |
| Rwork | 0.163 |
| R-free | 0.24031 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qnk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.664 |
| Data reduction software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.070 | 2.580 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.030 | 0.041 |
| Number of reflections | 8486 | |
| <I/σ(I)> | 17.2 | 3.1 |
| Completeness [%] | 89.7 | 60 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 40% (NH4)2SO4, Tris-HCl, 40mM MgCl2, 3% MPD, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
