3FDZ
Crystal structure of phosphoglyceromutase from burkholderia pseudomallei 1710b with bound 2,3-diphosphoglyceric acid and 3-phosphoglyceric acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-30 |
| Detector | SATURN |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.390, 48.480, 62.090 |
| Unit cell angles | 106.03, 91.54, 107.50 |
Refinement procedure
| Resolution | 19.750 - 2.250 |
| R-factor | 0.187 |
| Rwork | 0.183 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.526 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.310 | |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.071 | 0.345 |
| Number of reflections | 20934 | |
| <I/σ(I)> | 11.43 | 2.6 |
| Completeness [%] | 93.4 | 90.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 289 | 30% PEG 600, 5% PEG 1000, 10% GLYCEROL, 100 MM MES PH 6.0, CRYSTALS SOAKED OVERNIGHT WITH 20 MM 3-PHOSPHOGLYCERIC ACID, VAPOR DIFFUSION, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
