3FDX
Putative filament protein / universal stress protein F from Klebsiella pneumoniae.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-08 |
Detector | SBC-3 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.684, 67.684, 161.370 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.700 - 1.580 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.20900 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.945 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.700 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.050 | 0.590 |
Number of reflections | 58049 | |
<I/σ(I)> | 58.999 | 1.92 |
Completeness [%] | 97.2 | 77.1 |
Redundancy | 8.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.3 M magnesium formate, 0.1 M Bis-Tris buffer, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |