3FDX
Putative filament protein / universal stress protein F from Klebsiella pneumoniae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-08 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.684, 67.684, 161.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.700 - 1.580 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.20900 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.945 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.700 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.050 | 0.590 |
| Number of reflections | 58049 | |
| <I/σ(I)> | 58.999 | 1.92 |
| Completeness [%] | 97.2 | 77.1 |
| Redundancy | 8.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.3 M magnesium formate, 0.1 M Bis-Tris buffer, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
