3FDQ

Recognition of AT-rich DNA binding sites by the MogR Repressor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9790
Spacegroup name	P 1 21 1
Unit cell lengths	48.344, 93.597, 60.768
Unit cell angles	90.00, 113.41, 90.00

Refinement procedure

Resolution	50.000 - 1.750
R-factor	0.2169
Rwork	0.217
R-free	0.23220
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.101
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.740	1.740
Number of reflections	48556
Completeness [%]	97.1	64.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.0, 10-15% w/v PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MES
2	1	1	PEG 4000
3	1	2	MES
4	1	2	PEG 4000