3FDG
The crystal structure of the dipeptidase AC, Metallo peptidase. MEROPS family M19
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794, 0.9796 |
Spacegroup name | P 1 |
Unit cell lengths | 46.246, 50.683, 81.594 |
Unit cell angles | 82.42, 73.61, 68.18 |
Refinement procedure
Resolution | 78.330 - 1.800 |
R-factor | 0.17779 |
Rwork | 0.175 |
R-free | 0.22260 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.837 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.330 | 1.848 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.093 | 0.270 |
Number of reflections | 52476 | |
<I/σ(I)> | 16.48 | 2.44 |
Completeness [%] | 90.7 | 55.18 |
Redundancy | 2.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M Magnesium chloride hexahydrate, 0.1M Tris, 25% w/v Polyethylene glycol 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |