3FDF

Crystal structure of the serine phosphatase of RNA polymerase II CTD (SSU72 superfamily) from Drosophila melanogaster. Orthorhombic crystal form. Northeast Structural Genomics Consortium target FR253.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97926, 0.97949, 0.96791
Spacegroup name	P 21 21 2
Unit cell lengths	92.599, 157.282, 61.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 3.200
R-factor	0.252
Rwork	0.252
R-free	0.31300
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.089	0.447
Number of reflections	28511
<I/σ(I)>	26.1	5.6
Completeness [%]	98.8	100
Redundancy	13.8	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	PEG3350 18%, benzamidine 2%, KCl 0.2M, VAPOR DIFFUSION, HANGING DROP, temperature 297K