3FDD
The Crystal Structure of the Pseudomonas dacunhae Aspartate-Beta-Decarboxylase Reveals a Novel Oligomeric Assembly for a Pyridoxal-5-Phosphate Dependent Enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-01 |
Spacegroup name | I 2 3 |
Unit cell lengths | 150.453, 150.453, 150.453 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 106.600 - 2.350 |
R-factor | 0.17 |
Rwork | 0.168 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.102 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 106.600 | 2.410 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.057 | |
Number of reflections | 22531 | |
Completeness [%] | 100.0 | 99.2 |
Redundancy | 10.6 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 298 | ABDC crystals were grown at 23.5 C using the microbatch under-oil method by mixing in a 1:1 ratio, a 10.5 mg mL-1 ABDC solution in 50 mM Na-Acetate (pH 6.0) and 0.1 mM PLP with a solution containing 100 mM MgCl2, 100 mM Tris (pH 8.5), 25% (v/v) PEG 400, 10% (v/v) glycerol, temperature 298K, pH 7 |