3FDC
Crystal Structure of Avidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.279 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.200, 74.200, 85.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| Rwork | 0.255 |
| R-free | 0.33000 |
| Starting model (for MR) | 1ldo |
| Data reduction software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.100 |
| Rmerge | 0.099 |
| Number of reflections | 5233 |
| <I/σ(I)> | 28 |
| Completeness [%] | 99.9 |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 296 | 25% PEG 3000, 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 296.0K |
