3FD9
Crystal Structure of the transcriptional anti-activator ExsD from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-16 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 144.755, 69.347, 86.331 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.400 - 2.600 |
R-factor | 0.215 |
Rwork | 0.213 |
R-free | 0.26300 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.832 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | RESOLVE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.401 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.110 | 0.390 |
Number of reflections | 23616 | |
<I/σ(I)> | 1.7 | |
Completeness [%] | 92.5 | 57.4 |
Redundancy | 8 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 277 | 0.1 M Bis-Tris (pH 6.8), 0.2 M Ammonium Acetate, and 30% polyethylene glycol 3350 was combined at a 1:1 ratio with 20ExsD solution at 15 mg/ml in 25 mM Tris-HCl (pH 7.4), 0.15 M NaCl, 2 mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |