3FD8
Crystal Structure of an oxidoreductase from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.573, 151.321, 161.317 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.140 - 2.450 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.140 | 2.590 |
| High resolution limit [Å] | 2.450 | 2.440 |
| Rmerge | 0.147 | 0.480 |
| Number of reflections | 93942 | |
| <I/σ(I)> | 7.3 | 1 |
| Completeness [%] | 95.4 | 74.6 |
| Redundancy | 13.1 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Sodium Formate, 20% W/W Polyethylene Glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
