3FD4

Crystal Structure of Epstein-Barr virus gp42 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-26
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	64.792, 67.469, 79.463
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.730 - 2.400
R-factor	0.21666
Rwork	0.215
R-free	0.24226
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kg0
RMSD bond length	0.026
RMSD bond angle	1.708
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.730	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	14139
Completeness [%]	99.4	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	298	30% PEG-3000, 100 mM CHES pH 9.5, 10 mM YCl3, 25 mM tris pH 7.5, 150 mM NaCl, temperature 298K, VAPOR DIFFUSION