3FD3

Structure of the C-terminal domains of a LysR family protein from Agrobacterium tumefaciens str. C58.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97935,0.97948
Spacegroup name	P 43 21 2
Unit cell lengths	53.613, 53.613, 188.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.160 - 1.700
R-factor	0.171
Rwork	0.169
R-free	0.20000
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.381
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.074	0.054	0.405
Number of reflections	30842
<I/σ(I)>	51
Completeness [%]	98.3	99.6	78.4
Redundancy	14.8	13.9	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	295	0.1M sodium citrate pH5.6, 0.1M ammonium acetate, 15% PEG 4000, 1/800 (w/w) endoproteinase Glu-C V8, VAPOR DIFFUSION, temperature 295K