3FD2
Crystal structure of mMsoI/DNA complex with calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-25 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.932, 41.933, 70.960 |
| Unit cell angles | 107.21, 95.43, 109.44 |
Refinement procedure
| Resolution | 50.000 - 2.690 |
| R-factor | 0.215 |
| Rwork | 0.211 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m5x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.052 | 0.258 |
| Number of reflections | 11073 | |
| <I/σ(I)> | 21 | 4.51 |
| Completeness [%] | 97.0 | 95.8 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 291 | 22% PEG 400, 100 mM Tris pH 7.3, 10 mM calcium chloride, 20 mM sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
