3FD2
Crystal structure of mMsoI/DNA complex with calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Detector technology | IMAGE PLATE |
Collection date | 2008-03-25 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 |
Unit cell lengths | 41.932, 41.933, 70.960 |
Unit cell angles | 107.21, 95.43, 109.44 |
Refinement procedure
Resolution | 50.000 - 2.690 |
R-factor | 0.215 |
Rwork | 0.211 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m5x |
RMSD bond length | 0.008 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.052 | 0.258 |
Number of reflections | 11073 | |
<I/σ(I)> | 21 | 4.51 |
Completeness [%] | 97.0 | 95.8 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.3 | 291 | 22% PEG 400, 100 mM Tris pH 7.3, 10 mM calcium chloride, 20 mM sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |